17+ a modular cloning toolkit for genome editing in plants
An expanded cloning toolkit that contains 103 modules encoding a variety of CRISPRCas-based nucleases and their corresponding guide RNA backbones is presented that contains a set of. A Modular Cloning Toolkit for Genome Editing in Plants Florian Hahn1 Andrey Korolev12 Laura Sanjurjo Loures1 and Vladimir Nekrasov1 The Dawn.
A Golden Gate Modular Cloning Toolbox For Plants Acs Synthetic Biology
CRISPRCas9 Genome Editing Brochure.
. Find read and cite. The modular cloning kit presented in the study enables quick and facile assembly of DNA constructs for genome editing applications in plants and is an addition to previously. We believe the presented CRISPRCas toolkit is a great resource that will contribute towards wider adoption of the CRISPRCas genome editing technology and.
PDF Background CRISPRCas has recently become a widely used genome editing tool in various organisms including plants. The modular cloning MoClo based on the Golden Gate GG method has enabled development of cloning systems with standardised genetic parts eg.
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